I-Qrf Antibody Test Ishidi Elinganqunyiwe

Ukuhlolwa kwe-Qrf Antibody

Uhlobo: Ishidi elingasikiwe

Igama Brand: I-Bio-mapper

Ikhathalogi: REA0911

Isampula: WB/S/P

Ikhithi yokuhlola i-antibody pox virus isetshenziselwa ukuthola amasosha omzimba we-pox yezimvu ku-serum yezimvu, engasetshenziswa ukuhlola umphumela wokuzivikela komzimba womuthi wokugomela upokisi wezimvu noma ukusiza ekuhlonzweni kwesifo.Isimiso siwukuthi ikhithi yokuhlonza i-antibody virus ye-imvu pox yakhiwe ngepuleti le-antigen le-protein ye-imvu eboshwe ngaphambili, izimpawu ze-enzyme nezinye izinto ezisekelayo ezisekelayo, futhi isimiso se-enzyme-linked immunoassay (ELISA) sisetshenziselwa ukuthola i-antibody ye-imvu pox kusampula serum yezimvu.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo enemininingwane

Ukutholwa kwe-antibody yegciwane lemvu kwakhiwa i-microplate pre-coated ne-imvu pox nuclear protein antigen, izimpawu ze-enzyme namanye ama-reagents asekelayo, futhi isimiso se-enzyme-linked immunoassay (ELISA) sisetshenziselwa ukuthola i-antibody ye-pox yemvu kusampula serum yezimvu.Ngesikhathi sokuhlolwa, i-serum yokulawula kanye nesampula okufanele ihlolwe yengezwa epuleti le-microplate, futhi uma isampula iqukethe i-antibody pox yezimvu ngemva kokufukamela, izoboshelwa ku-antigen ku-microplate plate, nezinye izakhi ezingaboshiwe zizokhishwa ngemva kokugeza;Bese wengeza umaka we-enzayimu ukuze ubophezele ngokukhethekile ku-antigen-antibody complex ku-microplate plate;Omaka be-enzyme abangaboshiwe babe sebesuswa ngokuwashwa, futhi isixazululo se-TMB substrate sanezelwa emithonjeni, futhi umkhiqizo oluhlaza okwesibhakabhaka wakhiwa ukusabela kwe-conjugates ye-microplate, futhi ukujula kombala kwakuhlotshaniswa kahle nenani elithile lamasosha omzimba aqukethwe kusampula.Ngemuva kokuthi isisombululo sokuqeda sengezwe ukuze kuqedwe ukusabela, umkhiqizo waphenduka ophuzi;Inani lokumunca ekuphenduleni ngakunye linqunywa isifundi se-microplate kubude begagasi obungu-450 nm ukuze kutholwe ukuthi ingabe isampuli iqukethe amasosha omzimba wepokisi yezimvu.