I-Dengue fever yisifo esithathelwanayo esibangelwa igciwane lodenga, elidluliselwa kakhulu kubantu ngomiyane.Kudlangile emhlabeni wonke, kubangela izigidi zezifo kanye nezinkulungwane zokufa minyaka yonke.Izimpawu zomkhuhlane wodenga zihlanganisa imfiva ephezulu, ubuhlungu bekhanda, ubuhlungu bamalunga namalunga emisipha, futhi ezimweni ezimbi kakhulu, kungaholela ekopheni nasekulinyazweni kwezitho zomzimba.Ngenxa yokusakazeka kwayo ngokushesha nangokusakazeka kabanzi, imfiva yodenga iwusongo olukhulu empilweni yomphakathi nasenhlalakahleni yomhlaba wonke.
Ukuze kuhlolwe ngokushesha futhi kulawule ukubhebhetheka komkhuhlane wodenga, ukuhlolwa kwegciwane ngokushesha nangokunembile sekubalulekile.Mayelana nalokhu, izinsiza zokuxilonga ngokushesha zidlala indima ebalulekile.Angamathuluzi okuhlola asebenziseka kalula, asheshayo asiza izikhungo zezokwelapha nabaphenyi bezifo eziwumshayabhuqe ekutholeni ngokushesha ukuthi abantu ngabanye banalo yini igciwane lodenga.Ngokusebenzisa lezi zinsiza zokuxilonga, odokotela nabacwaningi bangakwazi ukuxilonga futhi bahlukanise abantu abanaleli gciwane kusenesikhathi, basebenzise izindlela ezifanele zokwelapha nokulawula, ngaleyo ndlela banqande ngempumelelo ukusakazeka kodenga.Ngakho-ke, izinsiza zokuxilonga ngokushesha zibambe ukubaluleka okukhulu ekuvimbeleni nasekulawuleni ukuqubuka komkhuhlane wodenga.
Isimiso Esisebenzayo kanye Nenqubo Yokusebenzisa Yekhithi Yokuxilonga Ngokushesha
· Izimiso eziyisisekelo zokusabela kwe-Antibody-Antigen
Ukusabela kwe-antibody-antigen kuwumgomo oyisisekelo ku-immunology esetshenziselwa ukuqashelwa okukhethekile kanye nokubopha ama-antigen.Amasosha omzimba abopha ama-antigen ukuze akhe izakhi zomzimba, inqubo ebophayo eqhutshwa ukukhangana kanye nokuhlobana phakathi kwamasosha omzimba nama-antigen.Esimweni sekhithi yokuhlola i-dengue fever, amasosha omzimba abopha ama-antigen avela egciwaneni lodenga, okuholela ekwakhekeni kwezakhi zokuzivikela zomzimba ezibonakalayo.
· Inqubo Yokuhlola Yekhithi Yokuxilonga
Isinyathelo 1: Letha isifanekiso nezingxenye zokuhlola ekamelweni lokushisa uma kuqandisiwe noma kuqandisiwe.Lapho inhlama incibilikile, hlanganisa kahle isifanekiso ngaphambi kokuhlolwa.
Isinyathelo sesi-2: Uma usulungele ukuhlola, vula isikhwama ku-notch bese ukhipha idivayisi.Beka idivayisi yokuhlola endaweni ehlanzekile, eyisicaba.
Isinyathelo sesi-3: Qiniseka ukuthi ulebula idivayisi ngenombolo ye-ID yesifanekiso.
Isinyathelo sesi-4: Ukuhlolwa kwegazi lonke
- Faka iconsi eli-1 legazi eliphelele (cishe u-30-35 µL) emthonjeni wesampula.
- Bese wengeza amaconsi ama-2 (cishe ama-60-70 µL) eDiluent Yesampuli ngokushesha.
Ngokuhlolwa kwe-serum noma i-plasma
- Gcwalisa i-pipette dropper ngesifanekiso.
- Ukubamba i-dropper ngokuqondile, khipha iconsi elingu-1 (cishe 30-35 µL) lesampula emthonjeni wesampula uqinisekisa ukuthi awekho amabhamuza omoya.
-Bese wengeza amaconsi ama-2 (cishe ama-60-70 µL) eDiluent Yesampuli ngokushesha.
Isinyathelo sesi-6: Imiphumela ingafundwa emizuzwini engama-20.Imiphumela emihle ingabonakala emfushane njengeminithi elingu-1.
Ungafundi imiphumela ngemva kwemizuzu engu-30. Ukuze ugweme ukudideka, lahla idivayisi yokuhlola ngemva kokuhumusha umphumela.
· Ukuhunyushwa komphumela wokuhlola
1. UMPHUMELA OMBI: Uma kuphela i-C band ithuthukiswa, ukuhlolwa kubonisa ukuthi izinga lodenga Ag esibonelweni alibonakali.Umphumela uba mubi noma awusebenzi.
2. UMPHUMELA OMHLE: Uma womabili amabhendi u-C no-T athuthukiswa, ukuhlolwa kubonisa ukuthi isifanekiso siqukethe udenga Ag.Umphumela uba omuhle noma uyasebenza.Amasampula anemiphumela emihle kufanele aqinisekiswe ngezinye izindlela zokuhlola ezifana ne-PCR noma i-ELISA kanye nokutholakele emitholampilo ngaphambi kokuthi kwenziwe isinqumo esihle.
3. OKUNGAvumelekile: Uma lingekho ibhendi C elithuthukisiwe, ukulinganisa akuvumelekile kungakhathaliseki ukuthi unjani umbala ku-T band njengoba kukhonjisiwe ngezansi.Phinda ukuhlola ngedivayisi entsha.
Izinzuzo ze-BoatBio Dengue Rapid Diagnostic Kit
· Ukushesha
1. Isikhathi Esincishisiwe Sokuhlola:
Ikhithi yokuxilonga isebenzisa ubuchwepheshe bokuhlola ngokushesha, okuvumela ukuhlaziya isampula kanye nokukhiqizwa kwemiphumela ukuthi kuqedwe kungakapheli imizuzu engama-20.
Uma kuqhathaniswa nezindlela zaselabhorethri ezivamile, ikhithi ifinyeza kakhulu isikhathi sokuhlola, ithuthukise ukusebenza kahle komsebenzi.
2. Ukuthola umphumela Wesikhathi Sangempela:
Ikhithi yokuxilonga inikeza imiphumela yesikhathi sangempela ngokushesha ngemva kokucubungula isampula nokuqedwa kokusabela.
Lokhu kwenza ochwepheshe bezokwelapha bakwazi ukwenza ngokushesha ukuxilonga nezinqumo, kusheshiswe ukuhlolwa kwezifo kanye nezinqubo zokwelashwa.
· Ukuzwela kanye nokucacisa
1. Ukuzwela Okuqinile:
Ukwakheka kwekhithi kuyenza ikwazi ukubona ukuba khona kwegciwane lodenga elizwela kakhulu.
Ngisho nasemasampula anokugxila kwegciwane okuphansi, ikhithi ithola igciwane ngokuthembekile, ithuthukisa ukunemba kokuxilonga.
2. Ukucaciswa okuphezulu:
Amasosha omzimba ekhithi akhombisa ukucaciswa okuphezulu, okuwavumela ukuthi ahlangane ngqo negciwane lodenga.
Leli khono lokuhlukanisa lenza ikhithi ikwazi ukuhlukanisa phakathi kokutheleleka ngegciwane lodenga namanye amagciwane ahlobene
(njengegciwane leZika, igciwane le-yellow fever), ukunciphisa ukuxilonga okungalungile kanye nemiphumela engemihle.
· Ukusebenzisa kalula
1. Izinyathelo Ezilula Zokusebenza:
Ikhithi yokuxilonga ngokuvamile ifaka izinyathelo zokusebenza eziqondile, ezivumela abasebenzisi ukuthi bazijwayeze ngokushesha nokusetshenziswa kwayo.
Izinyathelo ezicacile nezimfushane ziyabandakanyeka, okuhlanganisa ukungezwa kwesampula, ukuxutshwa kwe-reagent, ukusabela, nokuhumusha kwemiphumela.
2. Asikho Isidingo Sezisetshenziswa Eziyinkimbinkimbi noma Izimo Zelebhu:
Ikhithi yokuxilonga ngokuvamile ayidingi amathuluzi ayinkimbinkimbi noma izimo zelebhu ukuze isebenze nokufunda imiphumela.
Lokhu kuphatheka nokuvumelana nezimo kwenza ikhithi ifanelekele izimo ezihlukahlukene, okuhlanganisa izindawo ezikude noma izindawo zokunakekelwa kwezempilo ezinezinsiza ezilinganiselwe.
Kafushane, i-Dengue Rapid Diagnostic Kit inikeza izinzuzo ezifana nokushesha, ukuzwela, ukucacisa, kanye nokusebenziseka kalula, okuyenza ibe ithuluzi elibalulekile lokutholwa kwegciwane lodenga elisebenza kahle nelinembile kuzilungiselelo ezihlukahlukene.
Isincomo somkhiqizo
Isikhathi sokuthumela: Aug-16-2023