I-Filariasis IgG/IgM Rapid Test Kit (Colloidal Gold)

I-lymphatic filariasis eyaziwa ngokuthi i-Elephantiasis, ngokuyinhloko ebangelwa u-W. bancrofti no-B. malayi, ihlasela abantu abangaba izigidi ezingu-120 emazweni angaphezu kuka-80.Lesi sifo sidluliselwa kubantu ngokulunywa omiyane abanaleli gciwane lapho i-microflariae emuncwa esihlokweni somuntu onaso ikhula ibe izibungu zesigaba sesithathu.Ngokuvamile, ukuchayeka kaningi futhi isikhathi eside ezibungwini ezithelelekile kuyadingeka ukuze kusungulwe ukutheleleka kwabantu.

Ukuxilongwa kwe-parasitologic okuqondile ukubonakaliswa kwe-microflariae kumasampula egazi.Kodwa-ke, lokhu kuhlola okujwayelekile kwegolide kukhawulelwe imfuneko yokuqoqwa kwegazi ebusuku kanye nokuntuleka kokuzwela okwanele.Ukutholwa kwama-antigen ajikelezayo kuyatholakala ngokwentengiso.Ukusebenza kwayo kukhawulelwe ku-W. bancrofti.Ngaphezu kwalokho, i-microfilaremia ne-antigenemia ikhula kusukela ezinyangeni kuya eminyakeni ngemva kokuchayeka.

Ukutholwa kwe-antibody kunikeza izindlela kusenesikhathi zokuthola ukutheleleka kwe-filarial parasite.Ukuba khona kwe-IgM kuma-antigen e-parasite kuphakamisa ukutheleleka kwamanje, kanti, i-IgG ihambisana nesigaba sekwephuzile sokutheleleka noma ukutheleleka kwangaphambilini.Ngaphezu kwalokho, ukuhlonzwa kwama-antigen alondoloziwe kuvumela ukuhlolwa kwe-'pan-filaria' ukuthi kusebenze.Ukusetshenziswa kwama-recombinant amaprotheni kususa ukusabela okuphambene nabantu abanezinye izifo ze-parasitic.

I-Filariasis IgG/IgM Combo Rapid Test isebenzisa i-recombinant elondoloziwe

ama-antigen okuthola ngesikhathi esisodwa i-IgG ne-IgM ku-W. bancrofti kanye ne-B. malayi parasites ngaphandle kokuvinjelwa ekuqoqweni kwesifanekiso.

I-Filariasis IgG/IgM Rapid Test Kit iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-recombinant W. bancrofti kanye ne-B. malayi ama-antigen avamile ahlanganiswe negolide le-colloid (ama-Filariasis conjugates) kanye ne-rabbit IgG-gold conjugates, 2) i-nitrocellulose ye-nitrocellulose strip equkethe amabhande amabili okuhlola (i-C membrane kanye namabhande okuhlola amabili)Ibhendi engu-M imbozwe ngaphambilini nge-monoclonal anti-human IgM ukuze kutholwe i-IgM anti- W. bancrofti kanye ne-B. malayi, i-G band imbozwe ngaphambili ngama-reagents ukuze kutholwe i-IgG anti-W.i-bancrofti kanye ne-B. malayi, futhi i-C band ifakwe ngaphambili nge-anti-rabbit IgG yembuzi.

Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.W. bancrofti noma B. malayi Amasosha omzimba e-IgM uma ekhona kusifanekiso azobophezela kuma-conjugate e-Filariasis.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgM antibody, eyenza ibhande elingu-burgundy elingu-M, elibonisa umphumela wokuhlola we-W. bancrofti noma B. malayi IgM.

W. bancrofti noma B. malayi Amasosha omzimba e-IgG uma ekhona kusampula azobophezela kuma-conjugate e-Filariasis.I-immunocomplex bese ibanjwa ama-reagents ahlanganiswe ngaphambili kulwelwesi, enza ibhande le-G elinombala o-burgundy, okubonisa umphumela wokuhlola we-W. bancrofti noma B. malayi IgG.

Ukungabikho kwanoma yimaphi amabhendi okuhlola (M no-G) ​​kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi okuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.