I-Filariasis Antibody Rapid Test-kit (igolide le-Colloidal)

I-Filariasis Antibody Rapid Test-kit ( Colloidal Gold ) Isithombe Esifakiwe
  • I-Filariasis Antibody Rapid Test-kit (igolide le-Colloidal)
  • I-Filariasis Antibody Rapid Test-kit (igolide le-Colloidal)

INKCAZELO:Izivivinyo ezingama-25/ikhithi

UKUSEBENZISA OKUHLOSIWE:I-Filariasis Ab Rapid Test iyi-lateral flow immunoassay yokutholwa kwekhwalithi yamasosha omzimba ahlanganisa i-IgG, i-IgM, ne-IgA emithonjeni engaphansi ye-anti-lymphatic filarial parasites (W. Bancrofti no-B. Malayi) ku-serum yomuntu, i-plasma noma igazi lonke .Lokhu kuhlolwa kuhloswe ukuthi kusetshenziswe njengokuhlolwa kokuhlola futhi njengosizo ekuhlonzweni kwesifo se-Filariasis.Noma yisiphi isifanekiso esisebenzayo esine-Filariasis Ab Rapid Test kufanele siqinisekiswe ngenye indlela/izindlela zokuhlola.


Imininingwane Yomkhiqizo

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ISIFINYEZO KANYE NENCAZELO ISIVIVINYO

I-lymphatic filariasis eyaziwa ngokuthi i-Elephantiasis, ngokuyinhloko ebangelwa u-W. bancrofti no-B. malayi, ihlasela abantu abangaba izigidi ezingu-120 emazweni angaphezu kuka-80.Lesi sifo sidluliselwa kubantu ngokulunywa omiyane abanaleli gciwane lapho i-microflariae emuncwa esihlokweni somuntu onaso ikhula ibe izibungu zesigaba sesithathu.Ngokuvamile, ukuchayeka kaningi futhi isikhathi eside ezibungwini ezithelelekile kuyadingeka ukuze kusungulwe ukutheleleka kwabantu.

Ukuxilongwa kwe-parasitologic okuqondile ukubonakaliswa kwe-microflariae kumasampula egazi.Kodwa-ke, lokhu kuhlola okujwayelekile kwegolide kukhawulelwe imfuneko yokuqoqwa kwegazi ebusuku kanye nokuntuleka kokuzwela okwanele.Ukutholwa kwama-antigen ajikelezayo kuyatholakala ngokwentengiso.Ukusebenza kwayo kukhawulelwe ku-W. bancrofti.Ngaphezu kwalokho, i-microfilaremia ne-antigenemia ikhula kusukela ezinyangeni kuya eminyakeni ngemva kokuchayeka.

Ukutholwa kwe-antibody kunikeza izindlela kusenesikhathi zokuthola ukutheleleka kwe-filarial parasite.Ukuba khona kwe-IgM kuma-antigen e-parasite kuphakamisa ukutheleleka kwamanje, kanti, i-IgG ihambisana nesigaba sekwephuzile sokutheleleka noma ukutheleleka kwangaphambilini.Ngaphezu kwalokho, ukuhlonzwa kwama-antigen alondoloziwe kuvumela ukuhlolwa kwe-'pan-filaria' ukuthi kusebenze.Ukusetshenziswa kwama-recombinant amaprotheni kususa ukusabela okuphambene nabantu abanezinye izifo ze-parasitic.

I-Filariasis Ab Rapid Test isebenzisa ama-antigen ahlanganisiwe alondoloziwe ukuze ngesikhathi esisodwa ithole i-Antibody kuma-W. bancrofti kanye ne-B. malayi parasite ngaphandle kokuvinjelwa ekuqoqweni kwesifanekiso.

ISIMISO

I-Filariasis Ab Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-recombinant Filariasis ethize antigen ehlanganiswe negolide le-colloid (Filariasis conjugates) kanye ne-rabbit IgG-gold conjugates, 2) i-nitrocellulose membrane strip equkethe ibhendi yokuhlola (ibhendi ye-T) nesilawuli. ibhendi (C band).Ibhendi ye-T ihlanganiswe ngaphambili ne-un-conjugated Filariasis antigen, futhi ibhande C limbozwe ngaphambili nge-anti-rabbit IgG antibody.

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Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-AntiFilariasis Ab uma ikhona kusampula izobophezela kuma-conjugate we-Filariasis.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-antigen eboshwe ngaphambili, yakhe ibhande le-T elinombala obomvana, okubonisa umphumela wokuhlola we-aFilariasis Ab.Ukungabikho kwebhendi ye-T kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-anti-rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala ku-T band.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.

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