ISIFINYEZO KANYE NENCAZELO ISIVIVINYO
Amagciwane odenga, umndeni wama-serotypes amane ahlukene wamagciwane (Den 1,2,3,4), angamagciwane e-RNA agxilile, amboziwe, anomqondo omuhle.Amagciwane asakazwa omiyane bomndeni wakwaStegemyia abaluma emini, ikakhulukazi i-Aedes aegypti, kanye ne-Aedes albopictus.Namuhla, abantu abangaphezu kwezigidi eziyizinkulungwane ezingu-2,5 abahlala ezindaweni ezishisayo zase-Asia, e-Afrika, e-Australia naseMelika basengozini yokungenwa udenga.Izibalo ezilinganiselwa ezigidini eziyikhulu zomkhuhlane wodenga nezingu-250 000 zomkhuhlane wodenga osongela ukuphila zenzeka minyaka yonke emhlabeni wonke.
Ukutholwa kwe-Serological antibody ye-IgM kuyindlela evame kakhulu yokuxilongwa kwegciwane lodenga.Muva nje, ukutholakala kwama-antigen akhululwa ngesikhathi sokuphindaphinda kwegciwane esigulini esinegciwane kubonise umphumela othembisayo kakhulu.Inika amandla ukuxilongwa kusukela osukwini lokuqala ngemva kokuqala kwemfiva kuze kufike osukwini lwe-9, lapho isigaba somtholampilo sesifo sesiphelile, ngaleyo ndlela ivumela ukwelashwa kusenesikhathi ngokushesha4-.Ukuhlolwa Okushesha Kwe-Dengue NS1 kwenziwa ukuze kutholwe ukujikeleza kwe-antigen yodenga ku-serum. ,i-plasma noma igazi eliphelele.Ukuhlolwa kungenziwa ngabasebenzi abangaqeqeshiwe noma abanamakhono amancane, ngaphandle kwemishini yaselabhorethri.
ISIMISO
Ukuhlolwa Okushesha Kwe-Dengue NS1 kuyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-anti-dengue yegundane i-NS1 antigen ehlanganiswe negolide le-colloid (Dengue Ab conjugates), 2) i-nitrocellulose membrane strip equkethe ibhendi yokuhlola (T band) kanye nebhendi yokulawula (C iqembu).Ibhendi ye-T ifakwe ngaphambili ngegundane le-anti-dengue NS1 antigen, kanye nebhendi engu-C
ifakwe ngaphambili nge-anti-mouse IgG antibody.Amasosha omzimba e-antigen yodenga abona ama-antigen avela kuwo wonke ama-serotypes egciwane lodenga.
Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi lokuhlola.I-Dengue NS1 Ag uma ikhona kusifanekiso izobophezela kuma-conjugates we-Dengue Ab.I-immunocomplex ibe isithwetshulwa kulwelwesi i-antiNS1 yegundane eboshwe ngaphambili, yakhe ibhande le-T elinombala o-burgundy, okubonisa umphumela wokuhlolwa oqondile we-Dengue Ag.
Ukungabikho kwebhendi ye-T kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-anti-mouse IgG/mouse IgG-gold conjugate kungakhathaliseki ukuba khona kwebhendi ye-T enombala.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.
