Incazelo enemininingwane
I-Zika ixilongwa ngokusekelwe ekuhlaziyweni kwe-serological kanye nokuhlukaniswa kwegciwane kumagundane noma isiko lezicubu.I-IgM immunoassay iyindlela yokuhlola elebhu esebenza kakhulu.I-Zika IgM/IgG Rapid Test isebenzisa ama-antigen aphindayo atholakala kuphrotheni yesakhiwo sayo, ithola i-IgM/IgG anti-zika ku-serum yesiguli noma ku-plasma phakathi nemizuzu engu-15.Ukuhlolwa kungenziwa izisebenzi ezingaqeqeshiwe noma ezinamakhono amancane, ngaphandle kwemishini yaselabhorethri enzima.
I-Zika IgM/IgG Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe:
1) i-burgundy colored conjugate pad equkethe i-antigen recombinant ehlanganiswe negolide le-colloid (ama-Zika conjugates) kanye ne-rabbit IgG-gold conjugates,
2) umugqa we-nitrocellulose membrane oqukethe amabhendi amabili okuhlola (amabhendi angu-M no-G) kanye nebhendi yokulawula (ibhendi C).
Ibhendi engu-M imbozwe ngaphambili nge-IgM elwa nomuntu we-monoclonal ukuze kutholwe i-IgM elwa ne-Zika, ibhande le-G limbozwe ngaphambilini ngama-reagents ukuze kutholwe i-IgG anti-Zika, kanti ibhendi C imbozwe ngaphambili nge-anti-rabbit IgG yembuzi.