I-Zika IgG/IgM Rapid Test Kit (Colloidal Gold)

Igciwane leZika (Zika): lisakazwa kakhulu ngokulunywa umiyane i-Aedes, umama nengane, ukumpontshelwa igazi kanye nokuthelelana ngokocansi.I-antibody ye-IgG/IgM ikhiqizwa isonto elilodwa ngemva kokuqala, ngakho ukutholakala kwe-IgG/IgM kubaluleke kakhulu ekuqaleni.

ukuxilongwa kwe-Zika virus.I-Zika ixilongwa ngokusekelwe ekuhlaziyweni kwe-serological kanye nokuhlukaniswa kwegciwane kumagundane noma isiko lezicubu.I-IgM immunoassay iyindlela yokuhlola elebhu esebenza kakhulu.I-Zika IgM/IgG Rapid Test isebenzisa ama-antigen aphindayo atholakala kuphrotheni yesakhiwo sayo, ithola i-IgM/IgG anti-zika ku-serum yesiguli noma ku-plasma phakathi nemizuzu engu-15.Ukuhlolwa kungenziwa izisebenzi ezingaqeqeshiwe noma ezinamakhono amancane, ngaphandle kwemishini yaselabhorethri enzima.

I-Zika IgM/IgG Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-antigen recombinant conjugated ne-colloid gold (Zika conjugates) kanye namaconjugates anogwaja we-IgG-gold, 2) i-nitrocellulose membrane strip equkethe amabhendi amabili okuhlola (amabhendi we-M no-G) ​​kanye nesilawuli. ibhendi (C band).Ibhendi engu-M ifakwe ngaphambili nge-IgM elwa nomuntu ye-monoclonal ukuze kutholwe i-IgM elwa ne-Zika, ibhande le-G limbozwe ngaphambili ngama-reagents ukuze kutholwe i-IgG anti-Zika, kanti ibhendi engu-C imbozwe ngaphambilini nge-anti-Zika. unogwaja IgG.

Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-Anti-Zika IgM uma ikhona kusampula izobophezela kuma-conjugates e-Zika.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgM antibody, eyakha ibhendi engu-M enombala o-burgundy, okubonisa umphumela wokuhlola we-Zika IgM.

I-Anti-Zika IgG uma ikhona kusampula izobophezela kuma-conjugates e-Zika.I-immunocomplex ibe isithathwa ama-reagents ahlanganiswe ngaphambili kulwelwesi, enza ibhande le-G elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-Zika IgG.Ukungabikho kwanoma yimaphi amabhendi okuhlola (M no-G) ​​kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi okuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.