ISIFINYEZO KANYE NENCAZELO ISIVIVINYO
I-typhoid fever ibangelwa i-S. typhi, igciwane le-Gram-negative.Emhlabeni jikelele zilinganiselwa ezigidini eziyi-17 izehlakalo nokufa okuhlobene nazo eziyi-600,000 minyaka yonke.Iziguli ezitheleleke nge-HIV zisengozini enkulu kakhulu yokutheleleka nge-S. typhi.Ubufakazi bokutheleleka nge-H. pylori bubuye buveze ingozi eyengeziwe yokuthola i-typhoid fever.I-1-5% yeziguli iba umthwali wezifo ezingelapheki ophethe i-S. typhi kunyongo.
Ukuxilongwa komtholampilo kwe-typhoid fever kuncike ekuhlukaniseni kwe-S. typhi egazini, umnkantsha noma isilonda esithile se-anatomic.Ezikhungweni ezingakwazi ukwenza le nqubo eyinkimbinkimbi futhi edla isikhathi, ukuhlolwa kwe-Filix-Widal kusetshenziselwa ukwenza lula ukuxilongwa.Kodwa-ke, ukulinganiselwa okuningi kuholela ebunzimeni ekuchazeni ukuhlolwa kwe-Widal.
Ngokuphambene, i-Typhoid IgG/IgM Rapid Test Kit iwukuhlolwa kwaselabhorethri okulula futhi okusheshayo.Ukuhlola kanyekanye kuthola futhi kwehlukanise amasosha omzimba e-IgG kanye ne-IgM ku-S. typhi ethize antigen kusampula yegazi lonke ngaleyo ndlela kusize ekunqumeni ukuchayeka kwamanje noma kwangaphambilini ku-S. typhi.
ISIMISO
I-Typhoid IgG/IgM Combo Rapid Test i-lateral flow chromatographic
ukuhlolwa kwe-immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-recombinant S. typhoid H antigen kanye ne-O antigen ehlanganiswe negolide eli-colloid (ama-Typhoid conjugates) kanye nama-conjugate kanogwaja we-IgG-gold, 2) ulwelwesi lwe-nitrocellulose (i-C) ulwelwesi oluqukethe amabhande amabili okuhlola (M kanye nebhande le-G).Ibhendi ye-M ifakwe ngaphambili nge-monoclonal anti-human IgM ukuze kutholwe i-IgM anti-S.I-typhi, G band imbozwe ngaphambilini ngama-reagents ukuze kutholwe i-IgG
anti-S.typhi, futhi ibhande le-C limbozwe ngaphambili nge-anti unogwaja IgG.
Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.U-Anti-S.I-typhi IgM uma ikhona kusifanekiso izobophezela kuma-conjugate we-Typhoid.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgM antibody, eyakha ibhande le-M elinombala o-burgundy, okubonisa umphumela wokuhlolwa oqondile we-S. typhi IgM.
U-Anti-S.I-typhi IgG uma ikhona kusifanekiso izobophezela kuma-conjugate we-Typhoid.I-immunocomplex ibe isithathwa ama-reagents ahlanganiswe ngaphambili kulwelwesi, enza ibhande le-G elinombala obomvana, okubonisa umphumela wokuhlolwa omuhle we-S. typhi IgG.
Ukungabikho kwanoma yimaphi amabhendi okuhlola (M no-G) kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi okuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.
