Incazelo enemininingwane
I-Syphilis Tp iyi-bacterium ye-spirochete, okuyi-pathogen ye-venereal syphilis.Nakuba izinga lokutholakala kogcunsula e-United States lincipha ngemva kokugqashuka kogcunsula, izinga lokutholakala kogcunsula eYurophu belilokhu lenyuka lisuka ku-1986 laya ku-1991. Ngo-1992, kwanda izigameko ezingu-263, ikakhulukazi eRussian Federation.I-World Health Organization yabika abantu abasha abayizigidi ezingu-12 ngo-1995.
Ukutholwa ngokushesha kwenhlanganisela ye-syphilis antibody i-side flow chromatography immunoassay.
Ikhithi yokuhlola ihlanganisa: 1) i-recombinant Tp antigen IgG gold conjugate ehlanganisa purplish red conjugate pad colloidal gold (Tp conjugate) kanye onogwaja.
2) Ibhande le-Nitrocellulose membrane strip eliqukethe ibhendi yokuhlola (T) nebhendi yokulawula (ibhendi C).Ibhendi ye-T yayimbozwe ngaphambili nge-non-conjugate recombinant Tp antigen, futhi ibhande le-C lalimbozwe ngaphambili nge-anti-rabbit IgG antibody.
Uma ivolumu eyanele yesampula isatshalaliswa embobeni yesampula, isampula ithuthela kubhokisi ngesenzo se-capillary kubhokisi.Uma i-anti Tp antibody ikhona kusampula, izobophezela ku-Tp conjugate.Lesi sakhiwo sokuzivikela komzimba sibe sesithathwa kulwelwesi yi-antigen ye-Tp eboshwe ngaphambili, yakhe ibhande le-T elibomvu elibukhwebezane, okubonisa umphumela omuhle wokutholwa kwe-Tp antibody.Ukungabikho kwe-T band kubonisa ukuthi umphumela unegethivu.Ukuhlolwa okuhlanganisa nokulawula kwangaphakathi (i-band C) kufanele kubonise i-purple red band goat anti rabbit IgG/rabbit IgG gold conjugate ye-immune complex, kungakhathaliseki ukuthi i-T-band yayo.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi kufanele kusetshenziswe enye idivayisi.