Incazelo enemininingwane
Cabangela noma yiziphi izinto zemvelaphi yomuntu njengezithathelwanayo futhi uziphathe usebenzisa izinqubo ezijwayelekile ze-biosafety.
I-Plasma
1.Qoqa isifanekiso segazi eshubhuni le-lavender, eluhlaza okwesibhakabhaka noma eluhlaza okotshani (eliqukethe i-EDTA, i-citrate noma i-heparin, ngokulandelanayo ku-Vacutainer® ) ngokubhoboza imithambo.
2.Hlukanisa i-plasma nge-centrifugation.
3.Khipha ngokucophelela i-plasma uyifake eshubhuni elisha elilebulwe ngaphambilini.
I-Serum
1. Qoqa isifanekiso segazi eshubhuni eliqoqwe eliphezulu elibomvu (elingenawo ama-anticoagulant ku-Vacutainer®) ngokubhoboza imithambo.
2.Vumela igazi ukuthi lihlule.
3.Hlukanisa i-serum nge-centrifugation.
4.Khipha ngokucophelela i-serum uyifake eshubhuni elibhalwe ngaphambilini.
5.Hlola izifanekiso ngokushesha ngangokunokwenzeka ngemva kokuqoqa.Gcina izibonelo ku-2°C kuya ku-8°C uma zingahloliwe ngokushesha.
6.Gcina izibonelo ku-2°C kuya ku-8°C kufika ezinsukwini ezi-5.Ama-specimens kufanele aqandiswe ku -20°C ukuze agcine isikhathi eside
Igazi
Amaconsi egazi eliphelele angatholwa ngokubhoboza ithiphu leminwe noma ngokubhoboza imithambo.Ungasebenzisi noma yiliphi igazi eline-hemolized ukuhlola.Izibonelo zegazi eliphelele kufanele zigcinwe esiqandisini (2°C-8°C) uma zingahloliwe ngokushesha.Izifanekiso kufanele zihlolwe engakapheli amahora angama-24 eqoqwe. Gwema imijikelezo eminingi yokuqhwandisa.Ngaphambi kokuhlola, letha amasampula afriziwe ekamelweni lokushisa kancane futhi uhlanganise kahle.Izibonelo eziqukethe i-particle ebonakalayo kufanele zicaciswe nge-centrifugation ngaphambi kokuhlolwa.
INQUBO YOKUHLOLA
Isinyathelo 1: Letha isifanekiso nezingxenye zokuhlola ekamelweni lokushisa uma kuqandisiwe noma kuqandisiwe.Lapho inhlama incibilikile, hlanganisa kahle isifanekiso ngaphambi kokuhlolwa.
Isinyathelo sesi-2: Uma usulungele ukuhlola, vula isikhwama ku-notch bese ukhipha idivayisi.Beka idivayisi yokuhlola endaweni ehlanzekile, eyisicaba.
Isinyathelo sesi-3: Qiniseka ukuthi ulebula idivayisi ngenombolo ye-ID yesifanekiso.
Isinyathelo sesi-4: Ukuhlolwa kwegazi lonke – Faka ithonsi elilodwa legazi eliphelele (cishe u-30-35 µL) emthonjeni wesampula.– Bese wengeza amaconsi ama-2 (cishe ama-60-70 µL) eDiluent Yesampuli ngokushesha.