Ikhithi Yokuhlola Esheshayo I-Salmonella Typhoid Antigen Rapid (Colloidal Gold)

I-Enteric fever (i-typhoid kanye ne-paratyphoid fever) isifo esikhulu esibangelwa amagciwane.Nakuba lesi sifo singavamile emazweni athuthukile, siseyinkinga yezempilo ebalulekile neqhubekayo emazweni asathuthuka.Leyo fever ye-enteric iyinkinga enkulu yezempilo yomphakathi kulezo zifunda, ene-Salmonella enterica serovar typhi(Salmonella typhi) i-ejenti evame kakhulu ye-aetiologic kodwa enenani elikhulayo lezigameko ngenxa ye-Salmonella paratyphi.Ngenxa yokuthi izici eziyingozi ezifana nokungahlanzeki kahle kwendle, ukungabi khona kwamanzi okuphuza aphephile nezimo zomnotho eziphansi emazweni anezinsizakusebenza zikhuliswa ukuvela kwe-salmonellae emelana nezidakamizwa eziningi enokuncipha kokungenwa yi-fluoroquinolone, ehlotshaniswa nokwanda kokufa nokugula.

EYurophu, ukutheleleka kwe-Salmonella typhi kanye ne-Salmonella paratyphi kwenzeka phakathi kwabahambi ababuya ezindaweni ezivame ukugula.

I-Enteric fever ebangelwa i-Salmonella paratyphi ayibonakali i-fron ebangelwa i-Salmonella typhi.Lo mkhuhlane uvamise ukuvela isonto elilodwa kuya kwezintathu ngemuva kokuchayeka futhi uqunjelwa kakhulu.Izimpawu zihlanganisa ukushisa okukhulu, ubuthakathaka, ukukhathala, ubuhlungu bemisipha, ikhanda elibuhlungu, ukungakuthandi ukudla kanye nesifo sohudo noma ukuqunjelwa.Amabala abomvu avela esifubeni, ukuhlolwa ngokuvamile kuzoveza ukwanda kwesibindi nobende.Uma iseva inqamuka, kubikwe izimpawu zesimo sengqondo esishintshile kanye ne-meningitis (umkhuhlane, intamo eqinile, ukuquleka) kuye kwabikwa.

I-Salmonella Typhoid Antigen Rapid Test Kit iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-antigen recombinant ehlanganiswe negolide le-colloid (ama-monoclonal mouse anti-Salmonella Typhoid antibody conjugates) kanye ne-rabbit IgG-gold conjugates, 2) ibhande le-nitrocellulose eliqukethe ibhande lokuhlola (T) kanye nebhendi yokulawula (ibhendi C).Ibhendi ye-T imbozwe ngaphambili ngegundane elilwa ne-monoclonal anti-Salmonella Typhoid antibody ukuze kutholwe i-Salmonella Typhoid antigen, futhi i-C band imbozwe ngaphambili nge-anti-rabbit IgG yembuzi.Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.

I-Cryptosporidium uma ikhona kusifanekiso izobophezela kugundane le-monoclonal i-antiSalmonella Typhoid uma ikhona kusifanekiso izobophezela ku-monoclonal mouse antiSalmonella Typhoid antibody conjugates.I-immunocomplex ibe isithwetshulwa kulwelwesi yigundane eliboshwe ngaphambili elilwa ne-Salmonella Typhoid antibody, lenza ibhande le-T elinombala obomvana, okubonisa umphumela wokuhlolwa oqondile we-Salmonella Typhoid antigen.

Ukungabikho kwebhendi yokuhlola (T) kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi okuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile, futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.