I-RV IgG Ishidi Elinganqunywa Elisheshayo Lokuhlola

I-RV IgG Rapid Test Ishidi Elinganqunyiwe Elifakiwe Isithombe

Ukuhlolwa Okusheshayo kwe-RV IgG

Uhlobo: Ishidi elingasikiwe

Igama Brand: I-Bio-mapper

Ikhathalogi:RT0521

Isampula:WB/S/P

Ukuzwela: 91.10%

Ukucaciswa:99%

I-Rubella itholakala kakhulu ezinganeni ezifunda isikole kanye nentsha, futhi abantu abangaphezu kuka-80% banalo leli gciwane.Abesifazane abakhulelwe bangenwa igciwane le-rubella ngaphambi kwamasonto angu-20 okukhulelwa, futhi izehlakalo ze-teratogenesis ye-fetus zaziphezulu.Abantu abadala kanye nezingane abangenwe yi-rubella virus bangabangela ukuqubuka kwesikhumba.I-ELISA: Isithwali esimbozwe nge-antigen yegciwane le-rubella engacushiwe singabopha ne-antibody ethile kusampula esihloliwe, futhi i-antibody ehambisanayo itholwa nge-enzyme ebhalwe ukuthi i-anti human immunoglobulin kanye ne-substrate.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo enemininingwane

(1) Ukuze kuqoqwe isampula nokuhlolwa kokuhlolwa, isampula yegazi elilodwa kuphela elidinga ukuqoqwa.Kodwa-ke, uma kudingekile ukwahlulela isimo sokuzivikela somzimba sabantu abangenwe yigciwane, kuyadingeka ukuthatha amasampula ezigulini ezisolwayo ze-rubella zingakapheli izinsuku ezingu-3 ngemuva kokuqala kokuqubuka kanye nezinsuku eziyi-14 kuya kwezingu-21 ezilandelayo ukuze kutholwe kanyekanye.
(2) Ngokufanayo ne-ELISA evamile, engeza i-PBS 50 emgodini ngamunye wokulawula kanye nesampula μ l.Qhubeka wengeza isampula 10 μ l.Shisisa ku-25 ℃ imizuzu engu-45, geza futhi wome.
(3) Engeza omaka be-enzyme emthonjeni ngamunye 250 μ l.Indlela efanayo isetshenziselwa ukulondoloza ukushisa nokugeza.
(4) Engeza isixazululo se-pNPP substrate 250 μ l.Ngemva kokulondoloza ukushisa nokugeza ngendlela efanayo, engeza i-1mol/L i-sodium hydroxide 50 μ L Misa ukusabela, ulinganise inani lokumunca lembobo ngayinye ku-405nm, bese wahlulela umphumela wesampula esihloliwe.
(5) Uma kuwumphumela omuhle, isampula lingaphinda lihlanjululwe ukuze kunqunywe isihloko se-antibody, kuqhathanise imiphumela yamasampuli amabili alandelanayo, kanye nejaji.