Ikhithi Yokuhlola Esheshayo ye-Rotavirus Antigen (Igolide Le-Colloidal)

Isifo sohudo singenye yezimbangela eziyinhloko zokugula kwezingane nokufa kwabantu emhlabeni wonke, okuholela ekufeni kwabantu abayizigidi ezingu-2.5 ngonyaka.Ukutheleleka nge-Rotavirus kuyimbangela ehamba phambili yohudo olunzima ezinsaneni nasezinganeni ezineminyaka engaphansi kwemihlanu, okubalelwa ku-40% -60% we-acute gastroenteritis futhi kubangela ukufa kwezingane okulinganiselwa ku-500,000 unyaka ngamunye.Lapho eneminyaka emihlanu, cishe yonke ingane emhlabeni isingenwe yi-rotavirus okungenani kanye.Ngezifo ezilandelayo, impendulo ebanzi, ye-heterotypic antibody ikhishwa;ngakho-ke, abantu abadala abavamile ukuthinteka.

Kuze kube manje amaqembu ayisikhombisa ama-rotavirus (amaqembu AG) asehlukanisiwe futhi

uphawu.I-Rotavirus yeqembu A, i-rotavirus evame kakhulu, ibangela ngaphezu kwama-90% azo zonke izifo zeRotavirus kubantu.I-Rotavirus isakazwa ngokuyinhloko ngomzila we-fecaloral, ngokuqondile kusuka kumuntu kuya komuntu.Ama-virus titer esitokisini afinyelela umkhawulo ngemuva nje kokuqala kokugula, bese ehla.Isikhathi sokufukamela sokutheleleka nge-rotavirus kuvamise ukuba yizinsuku ezintathu kuya kwezintathu futhi silandelwa yi-gastroenteritis enesilinganiso sesikhathi sezinsuku ezintathu kuya kweziyisikhombisa.Izimpawu zalesi sifo zisukela ekuhudo okungatheni, okungamanzi kuye kohudo olunzima oluhambisana nomkhuhlane kanye nokuhlanza.

Ukuxilongwa kokutheleleka nge-rotavirus kungenziwa ngemuva kokuxilongwa kwe-gastroenteritis njengembangela yohudo olunzima ezinganeni.Muva nje, ukuxilongwa okuqondile kokutheleleka nge-rotavirus kuye kwatholakala ngokutholwa kwe-antigen yegciwane esitokisini ngezindlela ze-immunoassay ezifana ne-latex agglutination assay, i-EIA, kanye ne-lateral flow chromatographic immunoassay.

I-Rotavirus Ag Rapid Test iyi-lateral flow chromatographic immunoassay esebenzisa ipheya yamasosha omzimba athile ukuze ithole ngokufanelekile i-antigen ye-rotavirus esibonelweni se-fecal.Ukuhlolwa kungenziwa ngaphandle kwemishini yaselabhorethri enzima, futhi imiphumela iyatholakala kungakapheli imizuzu eyi-15.

I-Rotavirus Ag Rapid Test iyi-lateral flow chromatographic immunoassay.Umucu wokuhlola uqukethe: 1) i-burgundy colored conjugate pad equkethe i-monoclonal anti-rotavirus antibody ehlanganiswe negolide le-colloidal (ama-anti-rotavirus conjugates) kanye ne-anti-rotavirus conjugates) kanye ne-antibody elawulayo ehlanganiswe negolide le-colloidal, 2) i-nitrocellulose membrane strip equkethe umugqa wokuhlola (T umugqa) kanye nomugqa wokulawula (umugqa C).Ulayini we-T umbozwe ngaphambili nenye i-monoclonal anti-rotavirus, futhi umugqa u-C umbozwe ngaphambili nge-antibody yomugqa wokulawula.

Lapho ivolumu eyanele yesampula ekhishiwe ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-Rotavirus Ag, uma ikhona kusifanekiso, izobophezela kuma-conjugates e-anti-rotavirus.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-antibody ye-rotavirus ehlanganiswe ngaphambili eyakha umugqa we-T onemibala e-burgundy, okubonisa umphumela wokuhlola we-rotavirus. okubonisa umphumela ongemuhle we-rotavirus.Ukuhlolwa kuqukethe isilawuli sangaphakathi (umugqa C), okufanele sibonise umugqa onemibala e-burgundy we-immunocomplex yama-antibodies okulawula, kungakhathaliseki ukuthi ukuthuthukiswa kombala kulayini we-T.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.