Ikhithi Yokuhlola Esheshayo Ye-Mycoplasma Pneumoniae IgG/IgM (Colloidal Gold)

I-M.pneumoniae ingabangela inqwaba yezimpawu ezifana ne-primary atypical pneumonia, tracheobronchitis, nesifo somgudu wokuphefumula ophezulu.I-tracheobronchitis ivame kakhulu ezinganeni ezinezivikeli mzimba ezinciphile, futhi kuze kufike ku-18% wezingane ezingenwe yilesi sifo zidinga ukulaliswa esibhedlela.Ngokomtholampilo, i-M. pneumoniae ayikwazi ukuhlukaniswa nenyumoniya ebangelwa amanye amagciwane noma amagciwane.Ukuxilongwa okuqondile kubalulekile ngoba ukwelashwa kwe-M. pneumoniae nge-β-lactam antibiotics kungasebenzi kanti ukwelashwa ngama-macrolides noma i-tetracycline kunganciphisa isikhathi sokugula.

Ukunamathela kwe-M. pneumoniae ku-epithelium yokuphefumula kuyisinyathelo sokuqala senqubo yokutheleleka.Le nqubo yokunamathisela iwumcimbi oyinkimbinkimbi odinga amaprotheni amaningana e-adhesin, njenge-P1, i-P30, ne-P116.Isigameko sangempela sokutheleleka okuhlobene ne-M. pneumoniae asicacile njengoba kunzima ukuxilonga ezigabeni zokuqala zokutheleleka.

I-Mycoplasma Pneumoniae IgG/IgM Rapid Test Kit esekelwe kumgomo wekhwalithi ye-immunochromatographic assay ukuze kutholwe amasosha omzimba e-Mycoplasma Pneumoniae IgG/IgM ku-serum yomuntu, i-plasma noma igazi eliphelele. amasango), 2) i-nitrocellulose membrane strip equkethe ibhendi yokuhlola (i-T band) kanye nebhendi yokulawula (i-C band).Ibhendi ye-T ifakwe ngaphambili nge-anti-human IgG antibody, futhi ibhande le-C limbozwe ngaphambili nge-anti-mouse IgG antibody.I-Strip B iqukethe: 1) i-burgundy colored conjugate pad equkethe i-MP antigen ehlanganiswe negolide le-colloid (ama-MP Antigen conjugates), 2) umugqa we-nitrocellulose membrane equkethe ibhendi yokuhlola (i-T band) kanye nebhendi yokulawula (i-C band).Ibhendi ye-T ifakwe ngaphambili nge-anti-human IgM antibody, futhi ibhande le-C limbozwe ngaphambili nge-anti-mouse IgG antibody.

Isiqephu A:Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-antibody ye-MP IgG uma ikhona kusifanekiso izobophezela kuma-conjugate we-MP Antigen.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgG yegundane ehlanganiswe ngaphambili, yakhe ibhendi ye-T enombala obomvana, okubonisa umphumela wokuhlolwa oqondile we-MP IgG.Ukungabikho kwebhendi ye-T kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-anti-mouse IgG/mouse IgG-gold conjugate kungakhathaliseki ukuba khona kwebhendi ye-T enombala.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.

I-Strip B:Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-antibody ye-MP IgM uma ikhona kusifanekiso izobophezela kuma-conjugate we-MP Antigen.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgM yegundane ehlanganiswe ngaphambili, yakhe ibhendi ye-T enombala o-burgundy, ekhombisa umphumela wokuhlola we-MP IgM.Ukungabikho kwebhendi ye-T kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-anti-mouse IgG/mouse IgG-gold conjugate kungakhathaliseki ukuba khona kwebhendi ye-T enombala.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.