Ikhithi ye-Malaria Pf/Pan Antigen Rapid Test (Colloidal Gold)

Umalaleveva yisifo esithwalwa umiyane, i-hemolytic, i-febrile esihlasela abantu abangaphezu kwezigidi ezingama-200 futhi sibulala abantu abangaphezu kwesigidi esisodwa ngonyaka.Ibangelwa izinhlobo ezine zePlasmodium: P. falciparum, P. vivax, P. ovale, kanye ne-P. malariae.Le plasmodia yonke ingena futhi ibulale ama-erythrocyte abantu, ikhiqize ukugodola, imfiva, i-anemia, ne-splenomegaly.I-P. falciparum idala isifo esiyingozi kakhulu kunezinye izinhlobo ze-plasmodial futhi ibangela ukufa kwabantu abaningi umalaleveva.I-P. falciparum kanye ne-P. vivax yizifo ezivame kakhulu, nokho, kunokwehluka okukhulu kwendawo ekusabalaleni kwezinhlobo zezilwane.

Ngokwesiko, umalaleveva utholwa ngokuboniswa kwezinto eziphilayo ku-Giemsa ama-smear aminyene anegazi le-peripheral, futhi izinhlobo ezahlukene ze-plasmodium zihlukaniswa ngokubonakala kwazo kuma-erythrocyte anegciwane1.Inqubo iyakwazi ukuxilonga okunembayo nokuthembekile, kodwa kuphela lapho kwenziwa ochwepheshe be-microscope abanekhono besebenzisa imigomo echaziwe2, eveza izithiyo ezinkulu ezindaweni ezikude nezimpofu zomhlaba.

Ikhithi Yokuhlola Esheshayo Ye-Malaria Pf/Pan Antigen Rapid yenzelwe ukuxazulula lezi zithiyo.Ukuhlola kusebenzisa ipheya yamasosha omzimba e-monoclonal kanye ne-polyclonal ku-P. falciparum iphrotheni ethile, i-Histidine Repeat Protein II (pHRP-II), kanye nepheya lamasosha omzimba we-monoclonal ku-plasmodium Lactate Dehydrogenase (pLDH), iphrotheni ekhiqizwa izinhlobo ezine ze-plasmodium, ngaleyo ndlela ivumela ukutholakala kwe-Plasmodium ehlukile kanyekanye noma ngasikhathi sinye. i-odia.Ingenziwa ngabasebenzi abangaqeqeshiwe noma abanamakhono amancane, ngaphandle kwemishini yaselabhorethri.

Ikhithi yokuhlola esheshayo ye-Pf/Pan Malaria Rapid iyi-lateral flow chromatographic immunoassay.Izingxenye zomugqa wokuhlola zihlanganisa: 1) i-burgundy colored conjugate pad equkethe i-anti-pHRP-II antibody yegundane ehlanganiswe negolide eli-colloid (pHRP II-gold conjugates) kanye ne-mouse anti-pLDH antibody ehlanganiswe negolide eli-colloid (pLDH-gold conjugates),

2) i-nitrocellulose membrane strip equkethe amabhendi amabili okuhlola (amabhendi e-Pan ne-Pv) kanye nebhendi yokulawula (ibhendi C).Ibhendi ye-Pan imbozwe ngaphambili nge-monoclonal anti-pLDH antibody lapho ukutheleleka kwanoma yiziphi izinhlobo ezine ze-plasmodia kungatholwa, ibhande le-Pf limbozwe ngaphambili ngamasosha omzimba e-polyclonal anti-pHRP-II ukuze kutholwe ukutheleleka kwe-Pf, futhi ibhande C limbozwe nge-anti-mouse IgG yembuzi.

Ngesikhathi sokuhlolwa, ivolumu eyanele yesampula yegazi ikhishelwa emthonjeni wesampula (S) wekhasethi lokuhlola, isilondolozi se-lysis siyengezwa kumthombo we-buffer (B).Isigcinalwazi siqukethe okokuhlanza okulalisa amangqamuzana egazi abomvu futhi kukhiphe ama-antigen e-plasmodium ahlukahlukene, ahamba ngesenzo se-capillary emgqeni ogcinwe kukhasethi.I-pHRP-II uma iphrezentheshini kusifanekiso izobophezela ku-pHRP II-conjugates yegolide.I-immunocomplex ibe isithwetshulwa kulwelwesi ngamasosha omzimba ahlanganiswe ngaphambili ne-pHRPII, enza ibhande le-Pf elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-Pf.I-pLDH uma izipho kusifanekiso zizobophezela kuma-conjugate wegolide we-pLDH.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-pLDH antibody efakwe ngaphambili, yakhe ibhande lePan elinombala obomvana, okubonisa umphumela wokuhlola we-plasmodium.Uma ingekho ibhendi ye-Pan, kunganconywa umphumela wokuhlola omuhle wanoma iyiphi enye i-plasmodia emithathu.

Ukungabikho kwanoma yimaphi amabhendi okuhlola (i-Pan ne-Pf) kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-anti-mouse IgG / igundane IgG (pHRP-II kanye ne-pLDH-gold conjugates) kungakhathaliseki ukuthi umbala unjani kunoma yimaphi amabhendi okuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.