Ikhithi Yokuhlola Esheshayo I-Malaria Pf Antigen Rapid (Colloidal Gold)

Ikhithi Yokuhlola Esheshayo I-Malaria Pf Antigen Rapid (Colloidal Gold) Isithombe Esifakiwe
  • Ikhithi Yokuhlola Esheshayo I-Malaria Pf Antigen Rapid (Colloidal Gold)
  • Ikhithi Yokuhlola Esheshayo I-Malaria Pf Antigen Rapid (Colloidal Gold)

INKCAZELO:Izivivinyo ezingama-25/ikhithi

UKUSEBENZISA OKUHLOSIWE:I-Pf Ag Rapid Test iyi-lateral flow chromatographic immunoassay yokutholwa kwekhwalithi yephrotheni ethile ye-Plasmodium falciparum (Pf), i-Histidine-Rich Protein II (pHRP-II), kusampula yegazi lomuntu.Lo mshini uhloselwe ukuthi usetshenziswe njengokuhlolwa kokuhlola futhi njengosizo ekuxilongeni ukutheleleka nge-plasmodium.Noma isiphi isifanekiso esisebenzayo ne-Pf Ag Rapid Test kufanele siqinisekiswe ngezinye izindlela zokuhlola kanye nokutholakele emitholampilo.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

ISIFINYEZO KANYE NENCAZELO ISIVIVINYO

Umalaleveva yisifo esithwalwa umiyane, i-hemolytic, i-febrile esihlasela abantu abangaphezu kwezigidi ezingama-200 futhi sibulala abantu abangaphezu kwesigidi esisodwa ngonyaka.Ibangelwa izinhlobo ezine zePlasmodium: P. falciparum, P. vivax, P. ovale, kanye ne-P. malariae.Le plasmodia yonke ingena futhi ibulale ama-erythrocyte abantu, ikhiqize ukugodola, imfiva, i-anemia, ne-splenomegaly.I-P. falciparum ibangela isifo esiyingozi kakhulu kunezinye izinhlobo ze-plasmodial futhi ibangela ukufa kwabantu abaningi umalaleveva, futhi ingenye yezifo ezimbili ezivame kakhulu zikamalaleveva.

Ngokwesiko, umalaleveva utholwa ngokuboniswa kwezinto eziphilayo ku-Giemsa ama-smear aminyene anegazi le-peripheral, futhi izinhlobo ezahlukene ze-plasmodium zihlukaniswa ngokubonakala kwazo kuma-erythrocyte angenwe yilesi sifo.Inqubo iyakwazi ukuxilonga okunembayo nokuthembekile, kodwa kuphela uma kwenziwa osolwazi abanekhono besebenzisa izivumelwano ezichaziwe, ezethula izithiyo ezinkulu ezindaweni ezikude nezimpofu zomhlaba.

I-Pf Ag Rapid Test yakhelwe ukuxazulula lezi zithiyo.Ithola i-Pf ethize ye-antigen pHRP-II kusampula yegazi lomuntu.Ingenziwa ngabasebenzi abangaqeqeshiwe noma abanamakhono amancane, ngaphandle kwemishini yaselabhorethri.

ISIMISO

I-Pf Ag Rapid Test iyi-lateral flow chromatographic immunoassay.Izingxenye zomugqa wokuhlola zihlanganisa: 1) i-burgundy colored conjugate pad equkethe i-monoclonal anti- pHRP-II antibody ehlanganiswe negolide eline-colloid (pHRP II-gold conjugates), 2) i-nitrocellulose membrane strip equkethe ibhendi yokuhlola (Pf) kanye nebhendi yokulawula (C band).Ibhendi ye-Pf ifakwe ngaphambili ngamasosha omzimba e-polyclonal anti-pHRP-II, futhi ibhande le-C limbozwe ngaphambili nge-anti-mouse IgG.

asdhj

Ngesikhathi sokuhlolwa, ivolumu eyanele yesampula yegazi ikhishelwa emthonjeni wesampula (S) wekhasethi lokuhlola, isilondolozi se-lysis siyengezwa kumthombo we-buffer (B).Isigcinalwazi siqukethe okokuhlanza okulalisa amangqamuzana egazi abomvu futhi kukhiphe i-PHRPII antigen, enyakaza ngesenzo se-capillary emgqeni ogcinwe kukhasethi.I-pHRP-II uma iphrezentheshini kusifanekiso izobophezela ku-pHRP II-conjugates yegolide.I-immunocomplex ibe isithwetshulwa kulwelwesi ngamasosha omzimba e-polyclonal antipHRP II ahlanganiswe ngaphambili, enza ibhande le-Pf elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-Pf.

Ukungabikho kwebhendi ye-Pf kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-anti-mouse IgG / igundane IgG (pHRP II–gold conjugates) kungakhathaliseki ukuthi umbala unjani kunoma iyiphi ibhendi ye-Pf.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.

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