I-Leishmania IgG/IgM Rapid Test Kit (Igolide Elimhlophe)

I-Visceral leishmaniasis, noma i-Kala-azar, isifo esisatshalaliswayo esibangelwa izinhlobonhlobo ezimbalwa ze-L. donovani.Lesi sifo silinganiselwa ngabeWorld Health Organisation (WHO) ukuthi sihlasele abantu ababalelwa ezigidini ezingu-12 emazweni angu-88.Idluliselwa kubantu ngokulunywa yizimpukane ze-Phlebotomus, ezithola ukutheleleka ngokudla izilwane ezinesifo.Nakuba kuyisifo esitholakala emazweni ampofu, eNingizimu Yurophu, sekuyisifo esingosomathuba esihamba phambili ezigulini ezinengculaza.Ukuhlonzwa kwe-L. donovani organism kusuka egazini, umnkantsha, isibindi, ama-lymph nodes noma i-spleen kunikeza indlela eqondile yokuxilongwa.Ukutholwa kwe-serological ye-anti-L.I-donovani IgM itholakala iwumaka omuhle kakhulu we-acute Visceral leishmaniasis.Ukuhlola okusetshenziswa emtholampilo kuhlanganisa i-ELISA, i-fluorescent antibody noma ukuhlolwa okuqondile kwe-agglutination 4-5.Muva nje, ukusetshenziswa kwephrotheni ethile ye-L. donovani ekuhlolweni kuthuthukise ukuzwela nokucaciswa kwawo ngendlela emangalisayo.

I-Leishmania IgG/IgM Combo Rapid Test ukuhlola kabusha okusekwe kuphrotheni okusekelwe ku-serological, okuthola amasosha omzimba e-IgG ne-IgM ku-L. Donovani ngesikhathi esisodwa.Ukuhlolwa kunikeza umphumela onokwethenjelwa phakathi nemizuzu engu-15 ngaphandle kwanoma yiziphi izinsimbi.

I-Leishmania IgG/IgM Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-recombinant L. donovani antigen ehlanganiswe negolide eli-colloid (ama-conjugates e-Leishmania) kanye nama-conjugate kanogwaja we-IgG-gold, 2) umugqa we-nitrocellulose oqukethe amabhendi amabili okuhlola (amabhendi we-T1 kanye ne-T2) kanye nebhendi yokulawula (ibhendi C).Ibhendi ye-T1 imbozwe ngaphambili nge-monoclonal anti-human IgM ukuze kutholwe i-anti-L.I-donovani IgM, ibhendi ye-T2 ifakwe ngaphambili ngama-reagents ukuze kutholwe i-anti-L.i-donovani IgG, kanye nebhendi ye-C ifakwe ngaphambili nge-anti rabbit IgG.

Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-L. donovani IgM uma ikhona kusifanekiso izobophezela kuma-conjugate e-Leishmania.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgM antibody, eyakha ibhendi ye-T1 enombala obomvana, ebonisa umphumela wokuhlola we-L. donovani IgM.I-L. donovani IgG uma ikhona kusifanekiso izobophezela kuma-conjugate e-Leishmania.I-immunocomplex bese ibanjwa ama-reagents ahlanganiswe ngaphambili kulwelwesi, enze ibhande le-T2 elinombala obomvana, okubonisa umphumela wokuhlola we-L. donovani IgG.

Ukungabikho kwanoma yimaphi amabhendi e-T (T1 kanye ne-T2) kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi e-T.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.