Ukuhlolwa kwe-HIV / TP Antibody(Trilines)

I-HIV / TP Antibody Test(Trilines) Isithombe Esifakiwe

Ukuhlolwa kwe-HIV / TP Antibody(Trilines)

Uhlobo: Ishidi elingasikiwe

Igama Brand: I-Bio-mapper

Ikhathalogi:RC0211

Isampula:WB/S/P

Ukuzwela: 99.70%

Ukucaciswa:99.50%

Ukuhlola amapharamitha wobuchwepheshe okuthola i-treponema pallidum antibody (anti TP) kanye ne-AIDS virus antibody (anti-HIV 1/2) nge-DIGFA.Izindlela I-Sera yokulawula ikhwalithi eminingi kanye namasampula e-serum angu-5863 noma e-plasma eziguli atholwe ngamakhadi okuhlola e-DIGFA kanye ne-enzyme immunoassay (EIA) evela kubakhiqizi abathathu ngokulandelana.Ukuzwela, ukucaciswa, ukusebenza kahle kokutholwa kanye nezakhiwo ezibonakalayo zamakhadi okuhlola e-DIGFA kwahlolwa ngobuchwepheshe be-EIA njengereferensi.Imiphumela Ukucaciswa kwamakhadi okuhlola e-anti TP kanye ne-HIV 1/2 DIGFA ku-sera yokulawula ikhwalithi eminingi kwaba ngu-100%;Ukuzwela kwamakhadi okuhlola e-anti TP kanye ne-anti HIVI1/2DIGFA kwakungu-80.00% no-93.33% ngokulandelana;Ukusebenza kahle kokutholwa kwaba ngu-88.44% kanye nama-96.97% ngokulandelana.Ukucaciswa kwamakhadi okuhlola e-anti TP kanye ne-anti-HIV 1/2 DIGFA kumasampula e-serum (i-plasma) angu-5863 kwakungu-99.86% no-99.76% ngokulandelana;Ukuzwela kwaba ngu-50.94% no-77.78%, ngokulandelana;Ukusebenza kahle kokutholwa kwaba ngu-99.42% kanye nama-99.69% ngokulandelanayo.Isiphetho Ikhadi lokuhlola le-DIGFA linozwela oluphansi kanye nezindleko eziphezulu.Le nqubo ifanele ukuhlolwa kokuqala kweziguli eziphuthumayo, kodwa hhayi ukuhlolwa kokuhlolwa kwabanikela ngegazi.Uma isetshenziswa ekuhlolweni okusheshayo kwabanikela ngegazi emgwaqweni (imoto yokuqoqa igazi), kufanele kuhlanganiswe nobuchwepheshe be-EIA.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo enemininingwane

Indlela yokubona I ye-syphilis
Ukutholwa kwe-Treponema pallidum IgM antibody
Ukutholwa kwe-Treponema pallidum IgM antibody kuyindlela entsha yokuxilonga ugcunsula eminyakeni yakamuva.I-antibody ye-IgM iwuhlobo lwe-immunoglobulin, enezinzuzo zokuzwela okuphezulu, ukuhlonza kusenesikhathi, kanye nokunquma ukuthi umbungu ungenwe yini nge-Treponema pallidum.Ukukhiqizwa kwamasosha omzimba athile e-IgM kuwukusabela kokuqala komzimba okuhlekisayo komzimba ngemva kokutheleleka ngegcushuwa namanye amagciwane noma amagciwane.Ivamise ukuba ne-positive esigabeni sokuqala sokutheleleka.Iyanda ngokukhula kwesifo, bese i-antibody ye-IgG ikhuphuka kancane.
Ngemva kokwelashwa okuphumelelayo, i-antibody ye-IgM yanyamalala futhi amasosha omzimba e-IgG aphikelela.Ngemuva kokwelashwa kwe-penicillin, i-TP IgM yanyamalala esigabeni sokuqala iziguli ze-syphilis ezine-TP IgM positive.Ngemuva kokwelashwa kwe-penicillin, iziguli ze-TP IgM ezine-syphilis yesibili zanyamalala phakathi nezinyanga ezimbili kuya kweziyisi-8.Ngaphezu kwalokho, ukutholwa kwe-TP IgM kubaluleke kakhulu ekuxilongweni kwe-syphilis yokuzalwa ezinganeni ezisanda kuzalwa.Ngenxa yokuthi i-molecule ye-IgM antibody inkulu, i-antibody ye-IgM kamama ayikwazi ukudlula ku-placenta.Uma i-TP IgM ine-positive, ingane inegciwane.
Indlela yokuthola ugcunsula II
Ukutholwa kwebhayoloji yamangqamuzana
Eminyakeni yamuva nje, i-biology yamangqamuzana ithuthuke ngokushesha, futhi ubuchwepheshe be-PCR buye basetshenziswa kabanzi emisebenzini yomtholampilo.Okubizwa ngokuthi i-PCR i-polymerase chain reaction, okungukuthi, ukukhulisa ukulandelana kwe-spirochete DNA ekhethiwe kusuka ezintweni ezikhethiwe, ukuze kwandiswe inani lamakhophi e-DNA e-spirochete ekhethiwe, engenza kube lula ukutholakala ngama-probes athile, futhi kuthuthukise izinga lokuxilonga.
Kodwa-ke, le ndlela yokuhlola idinga ilabhorethri enezimo ezinhle ngokuphelele kanye nochwepheshe besigaba sokuqala, futhi kukhona amalabhorethri ambalwa anezinga eliphezulu kangaka eShayina okwamanje.Uma kungenjalo, uma kukhona ukungcola, uzobeka i-Treponema pallidum, futhi ngemva kokukhulisa i-DNA, kuyoba ne-Escherichia coli, okukwenza udabuke.Eminye imitholampilo emincane ivamise ukulandela imfashini.Balenga uhlobo lwelabhorethri ye-PCR futhi badle futhi baphuze ndawonye, okungaba ukuzikhohlisa kuphela.Eqinisweni, ukuxilongwa kwe-syphilis akudingi i-PCR, kodwa ukuhlolwa kwegazi okujwayelekile.