Incazelo enemininingwane
Uma kukhona inani elithile le-HIV-1 antibody noma i-HIV-2 antibody kuserum, i-antibody ye-HIV ku-serum kanye ne-antigen ye-gp41 ephinde yahlangana kanye ne-gp36 antigen ekulebula eligolide kuzovalwa amasosha omzimba ukuze akhe inkimbinkimbi lapho i-chromatography ifika endaweni yelebula legolide.Lapho i-chromatography ifinyelela emugqeni wokuhlola (umugqa we-T1 noma umugqa we-T2), inkimbinkimbi izokwenziwa i-immunoconjugated ne-antigen ye-gp41 ephinde ishumekwe kulayini we-T1 noma i-antigen ye-gp36 ephinde ishumekwe kulayini we-T2, ukuze igolide le-colloidal lifakwe umbala kumugqa we-T1 noma umugqa we-T2.Lapho amalebula egolide asele eqhubeka nokufakwa nge-chromatographed emugqeni wokulawula (umugqa C), ilebula yegolide izofakwa umbala ngokusabela kwamasosha omzimba nge-multiantibody eshumekwe lapha, okungukuthi, kokubili umugqa ongu-T kanye nomugqa ongu-C kuzoba nombala njengamabhande abomvu, okubonisa ukuthi amasosha omzimba e-HIV aqukethwe egazini;Uma i-serum ingenawo ama-antibody e-HIV noma ingaphansi kwenani elithile, i-antigen ye-gp41 noma i-gp36 antigen ku-T1 noma i-T2 ngeke isabele, futhi umugqa we-T ngeke ubonise umbala, kuyilapho i-polyclonal antibody ku-C line izobonisa umbala ngemva kokusabela kwamasosha omzimba ngelebula legolide, okubonisa ukuthi alikho i-antibody ye-HIV egazini.