Ukuhlolwa kwe-HIV/HCV Antibody(Trilines)

I-HIV/HCV Antibody Test(Trilines) ishidi elingasikiwe:

Uhlobo:Ishidi elingasikiwe

Ikhathalogi:RC0111

Isampula:WB/S/P

Ukuzwela: 99.70%

Ukucaciswa:99.80%

Amasosha omzimba we-AIDS angamelana ngempumelelo negciwane le-AIDS.I-antibody ye-Hepatitis C Igama lesiNgisi: HCV Ab ukutheleleka okungapheli kwegciwane le-hepatitis C (HCV) kungaholela ekuvuvukeni okungapheli, i-necrosis kanye ne-fibrosis yesibindi.Ezinye iziguli zingakhula zibe yi-cirrhosis ngisho ne-hepatocellular carcinoma (HCC), eyingozi kakhulu empilweni nasempilweni yeziguli, futhi isibe yinkinga enkulu yezenhlalo neyomphakathi.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo enemininingwane

Izindlela ezijwayelekile zokuthola ama-antibody e-AIDS yilezi:
1. Ukutholwa kwe-pathogen
Ukutholwa kwe-pathogen ikakhulukazi kubhekisela ekutholweni okuqondile kwamagciwane noma izakhi zofuzo ezibangelwa amagciwane ngokuhlukaniswa kwegciwane nesiko, ukubhekwa kwe-electron microscopic morphology, ukutholwa kwe-antigen yegciwane kanye nokunqunywa kofuzo.Izindlela ezimbili zokuqala zinzima futhi zidinga imishini ekhethekile kanye nochwepheshe abaqeqeshiwe.Ngakho-ke, ukutholwa kwe-antigen kuphela kanye ne-RT-PCR (i-PCR yokubhalwa kwe-reverse) okungasetshenziselwa ukuxilongwa komtholampilo.
2. Ukutholwa kwe-antibody
I-antibody ye-HIV ku-serum iyinkomba engaqondile yokutheleleka nge-HIV.Ngokobubanzi bayo obuyinhloko bokusebenza, izindlela ezikhona zokuhlonza amasosha omzimba we-HIV zingahlukaniswa ngokuhlolwa kokuhlolwa nokuhlolwa kokuqinisekisa.
3. I-reagent yokuqinisekisa
I-Western blot (WB) iyindlela evame ukusetshenziswa kakhulu yokuqinisekisa i-serum enhle yokuhlolwa kokuhlolwa.Ngenxa yesikhathi sayo sewindi eside uma kuqhathaniswa, ukungezwani kahle kanye nezindleko eziphakeme, le ndlela ifaneleka kuphela ukuhlolwa kokuqinisekisa.Ngokuthuthuka kokuzwela kwesizukulwane sesithathu nesine sesizukulwane sesine se-HIV, i-WB isilokhu ingakwazi ukuhlangabezana nezidingo zokusetshenziswa kwayo njengokuhlola okuqinisekisayo.
Olunye uhlobo lwe-reagent yokuqinisekisa yokuhlola egunyazwe yi-FDA i-immunofluorescence assay (IFA).I-IFA ibiza ngaphansi kwe-WB, futhi ukusebenza kulula.Yonke inqubo ingaqedwa phakathi namahora angu-1-1.5.Ububi obuyinhloko bale ndlela ukuthi idinga izitholi ze-fluorescence ezibizayo kanye nochwepheshe abanolwazi ukuze babheke imiphumela yokuhlola, futhi imiphumela yokuhlola ayikwazi ukugcinwa isikhathi eside.Manje i-FDA incoma ukuthi imiphumela ye-IFA engemihle noma emihle kufanele ibe khona lapho ikhipha imiphumela yokugcina kubanikeli i-WB yabo engenakunqunywa, kodwa akubhekwa njengendinganiso yokufaneleka kwegazi.
4. Ukuhlolwa kokuhlola
Ukuhlolwa kokuhlola kusetshenziswa kakhulu ukuhlola abanikela ngegazi, ngakho kudinga ukusebenza okulula, izindleko eziphansi, ukuzwela kanye nokucaciswa.Njengamanje, indlela yokuhlola eyinhloko emhlabeni kuseyi-ELISA, futhi kukhona ama-reagents e-particle agglutination ambalwa kanye nama-reagents e-ELISA asheshayo.
I-ELISA inokuzwela okuphezulu nokucacile, futhi kulula ukuyisebenzisa.Ingasetshenziswa kuphela uma ilabhorethri ifakwe isifundi se-microplate kanye newasha yamapuleti.Ifaneleka ikakhulukazi ekuhlolweni okukhulu elabhorethri.
Ukuhlolwa kwe-particle agglutination kungenye indlela elula, elula futhi engabizi kakhulu yokuthola.Imiphumela yale ndlela ingahlulelwa ngamehlo anqunu, futhi ukuzwela kuphezulu kakhulu.Ifaneleka ikakhulukazi emazweni asathuthuka noma inani elikhulu labanikela ngegazi.Okubi ukuthi amasampula amasha kumele asetshenziswe, futhi ukucaciswa kwawo akulungile.
Umtholampilo we-antibody we-Hepatitis C virus:
1) I-80-90% yeziguli ezinesifo sokusha kwesibindi ngemva kokumpontshelwa igazi ziyi-hepatitis C, iningi lazo lihle.
I-2) Ezigulini ezine-hepatitis B, ikakhulukazi lezo ezivame ukusebenzisa imikhiqizo yegazi (i-plasma, igazi eliphelele) ingabangela ukutheleleka kwe-co ye-hepatitis C, okwenza lesi sifo sibe esingamahlalakhona, i-cirrhosis yesibindi noma umdlavuza wesibindi.Ngakho-ke, i-HCV Ab kufanele itholwe ezigulini ezine-hepatitis B ephindaphindiwe noma iziguli ezine-hepatitis engapheli.