ISIFINYEZO KANYE NENCAZELO ISIVIVINYO
I-Helicobacter pylori ihlotshaniswa nezifo ezihlukahlukene ze-gastrointestinal ezihlanganisa i-non-ulcer dyspepsia, izilonda ze-duodenal ne-gastric kanye ne-gastritis esebenzayo, engapheli.Ukuvama kokutheleleka kwe-H. pylori kungadlula ama-90% ezigulini ezinezimpawu nezimpawu zezifo zesisu.Ucwaningo lwakamuva lubonisa ukuhlotshaniswa kokutheleleka kwe-H. pylori nomdlavuza wesisu.
I-pylori ingadluliselwa ngokungenisa ukudla noma amanzi angcoliswe i-fecal matter.Ama-antibiotics ahlanganiswe ne-bismuth compounds aboniswe ukuthi ayasebenza ekwelapheni ukutheleleka kwe-H. Pylori okusebenzayo..H.ukutheleleka kwe-pylori okwamanje kutholwa ngezindlela zokuhlola ezihlaselayo ezisekelwe ku-endoscopy ne-biopsy (okungukuthi, i-histology, isiko) noma izindlela zokuhlola ezingahlaseli ezifana ne-urea breath test (UBT), ukuhlolwa kwe-serologic antibody kanye nokuhlolwa kwe-stool antigen.I-UBT idinga okokusebenza kwelebhu ebizayo kanye nokusetshenziswa kwe-reagent enemisebe.Ukuhlolwa kwe-antibody ye-Serologic akuhlukanisi phakathi kwezifo ezikhona manje kanye nokuchayeka okwedlule noma ukutheleleka osekulashiwe.I-stool antigen test ithola i-antigen ekhona endle, ekhombisa ukutheleleka okusebenzayo kwe-H. pylori.Ingase futhi isetshenziselwe ukuqapha ukusebenza ngempumelelo kokwelashwa kanye nokuphinda kwenzeke kokutheleleka.I-H. pylori Ag Rapid Test isebenzisa i-colloidal gold conjugated monoclonal anti-H.i-pylori antibody kanye nenye i-monoclonal anti-H.i-pylori antibody ukuze ithole ngokuqondile i-H. pylori antigen ekhona esibonelweni se-fecal yesiguli esithelelekile.Ukuhlolwa kuyasebenziseka kalula, kunembile, futhi umphumela utholakala phakathi nemizuzu engu-15.
ISIMISO
I-H. pylori Ag Rapid Test iyisemishi i-lateral flow chromatographic immunoassay.Umucu wokuhlola uqukethe: 1) iphedi ye-conjugate enemibala e-burgundy equkethe i-monoclonal anti-H.i-pylori antibody ehlanganiswe negolide le-colloidal (ama-anti-Hp conjugates) kanye no-2) umugqa we-nitrocellulose membrane oqukethe umugqa wokuhlola (umugqa we-T) kanye nolayini wokulawula (umugqa C).Ulayini we-T uhlanganiswe ngaphambili nenye i-monoclonal anti-H.i-pylori antibody, kanye nomugqa ongu-C umbozwe ngaphambili nge-anti-mouse IgG antibody.
Lapho ivolumu eyanele yesampula ye-fecal ekhishiwe ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kukhasethi.Ama-antigens e-H. pylori, uma ekhona esibonelweni, azobophezela kuma-anti-Hp conjugates.I-immunocomplex ibe isithathwa kulwelwesi yi-antibody eboshwe ngaphambili eyenza umugqa we-T burgundy onemibala, okubonisa umphumela wokuhlola we-H. pylori.Ukungabi bikho komugqa we-T kuphakamisa ukuthi ukugcwala kwama-antigen e-H. pylori kusifanekiso kungaphansi kwezinga elibonakalayo, okubonisa umphumela wokuhlola ongemuhle we-H. pylori. Ukuhlola kuqukethe isilawuli sangaphakathi (umugqa C) okufanele sibonise umugqa onemibala e-burgundy i-immunocomplex yembuzi ephikisana negundane IgG/mouse IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kulayini T.Uma ulayini u-C ungathuthuki, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.
