ISIFINYEZO KANYE NENCAZELO ISIVIVINYO
Amagciwane odenga, umndeni wama-serotypes amane ahlukene wamagciwane (Den 1,2,3,4), angamagciwane e-RNA agxilile, amboziwe, anomqondo omuhle.Amagciwane asakazwa omiyane bomndeni wakwaStegemyia abaluma emini, ikakhulukazi i-Aedes aegypti, kanye ne-Aedes albopictus.Namuhla, abantu abangaphezu kwezigidi eziyizinkulungwane ezingu-2,5 abahlala ezindaweni ezishisayo zase-Asia, e-Afrika, e-Australia naseMelika basengozini yokungenwa udenga.Izibalo ezilinganiselwa ezigidini eziyi-100 zomkhuhlane wodenga kanye nezingu-250,000 zomkhuhlane wodenga osongela ukuphila zenzeka minyaka yonke emhlabeni wonke1-3.
Ukutholwa kwe-Serological antibody ye-IgM kuyindlela evame kakhulu yokuxilongwa kwegciwane lodenga.Muva nje, ukutholakala kwama-antigen akhululwa ngesikhathi sokuphindaphinda kwegciwane esigulini esinegciwane kubonise umphumela othembisayo kakhulu.Inika amandla ukuxilongwa kusukela osukwini lokuqala ngemva kokuqala kwemfiva kuze kufike osukwini lwe-9, uma isigaba somtholampilo sesifo sesiphelile, ngaleyo ndlela kuvumela ukwelashwa kusenesikhathi endaweni ngokushesha4-.Ukuhlolwa Okusheshayo Kwe-Dengue IgG/IgM kuthuthukiswa ukuze kutholwe ukujikeleza kwe-antigen yodenga ku-serum, i-plasma noma igazi lonke.Ukuhlolwa kungenziwa ngabasebenzi abangaqeqeshiwe noma abanamakhono amancane, ngaphandle kwemishini yaselabhorethri.
ISIMISO
I-Dengue IgG/IgM Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe ama-antigen emvilophu enziwe kabusha yodenga ahlanganiswe negolide eli-colloid (ama-dengue conjugates) nama-conjugate kanogwaja we-IgG-gold,2) ulwelwesi lwe-nitrocellulose oluqukethe amabhendi amabili okuhlola (ibhendi ye-G kanye ne-C) nokulawula ibhande).I-G band imbozwe ngaphambili ne-antibody ukuze kutholwe igciwane le-IgG elilwa nodenga, i-M band imbozwe ngamasosha omzimba ukuze kutholwe igciwane le-IgM elilwa nodenga, kanti ibhande C limbozwe ngaphambili nge-anti unogwaja IgG.
Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.Igciwane le-IgG elilwa nodenga uma likhona kusampula lizobophezela kuma-conjugates odenga.I-immunocomplex bese ibanjwa i-reagent ehlanganiswe ku-G band, yenza ibhande le-G elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-virus we-dengue IgG futhi iphakamisa ukutheleleka kwakamuva noma okuphindaphindiwe.Igciwane le-IgM elilwa nodenga, uma likhona kusampula, lizobophezela kuma-conjugates odenga.I-immunocomplex ibe isithathwa yi-reagent embozwe ngaphambili ku-M band, yakhe ibhande le-M elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-IgM virus we-dengue virus futhi iphakamisa ukutheleleka okusha.
Ukungabi bikho kwanoma yimaphi amabhendi okuhlola (G kanye no-M) kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma imaphi amabhendi e-T.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.
