Incazelo enemininingwane
Ikhithi yokuhlola esheshayo ye-Canine parvovirus antigen isebenzisa umgomo wendlela yesemishi ye-antibody ukuze ithole ngokufanelekile i-canine parvovirus antigen endle yenja.I-antibody 1 yenja evamile ye-parvovirus yasetshenziswa njengomaka, futhi indawo yokuthola (T) kanye nesifunda sokulawula (C) kulwelwesi lwe-nitrocellulose zambozwa nge-canine parvovirus antibody 2 kanye ne-anti-chicken yezimvu, ngokulandelana.Ngesikhathi sokutholwa, isampula i-chromatographic ngaphansi kwemiphumela ye-capillary.Uma isampula elihloliwe liqukethe i-canine parvovirus antigen, i-antibody standard 1 yakha i-antigen-antibody complex ene-canine parvovirus, futhi ihlanganisa ne-canine parvovirus antibody 2 egxilwe endaweni yokuhlonza ngesikhathi se-chromatography ukuze kwakhe isangweji “ye-antibody 1-antigen-antibody 2″, okuholela endaweni etholakala ngokunsomi-bomvu (T);Ngokuphambene, awekho amabhendi abomvu-onsomi avela endaweni yokuthola (T);Kungakhathalekile ukuba khona noma ukungabikho kwe-canine parvovirus antigen kusampula, inhlanganisela ye-IgY yenkukhu evamile esagolide izoqhubeka nokubekwa phezulu endaweni yokulawula (C), futhi kuzovela ibhendi ebomvu-purplish.Ibhendi ebomvu-nsomi ethulwa endaweni yokulawula (C) iyindinganiso yokwahlulela ukuthi inqubo ye-chromatography ivamile yini, futhi isebenza njengezinga lokulawula langaphakathi lama-reagents.