ISIFINYEZO KANYE NENCAZELO ISIVIVINYO
Ikholera yisifo esithathelwanayo esibonakala ngokulahleka okukhulu koketshezi lomzimba nama-electrolyte ngenxa yohudo olunzima.I-etiological ejenti yekholera ikhonjwe njenge-Vibrio cholerea (V. Cholerae), i-bacterium eyi-gram negative, evame ukudluliselwa kubantu ngamanzi nokudla okungcolile.
Uhlobo lwe-V. Cholerae luhlukaniswe ngama-serogroups amaningana ngesisekelo se-O antigens.Amaqembu amancane i-O1 kanye ne-O139 anentshisekelo ekhethekile ngoba womabili angabangela ubhubhane kanye nekholera ewubhubhane.Kubalulekile ukunquma ngokushesha ngangokunokwenzeka ukuba khona kwe-V. cholerae O1 kanye ne-O139 kumasampula omtholampilo, emanzini, nokudla ukuze kuqashwe okufanele kanye nezinyathelo zokuvimbela eziphumelelayo zithathwe iziphathimandla zezempilo zomphakathi.
I-Cholera Ag Rapid Test ingasetshenziswa ngokuqondile emkhakheni ngabasebenzi abangaqeqeshiwe noma abanamakhono amancane futhi umphumela utholakala ngaphansi kwemizuzu eyi-10, ngaphandle kwemishini yaselabhorethri enzima.
ISIMISO
I-Cholera Ag Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-monoclonal anti-V.Amasosha omzimba ekholera O1 kanye ne-O139 ahlanganiswe negolide le-colloid (ama-conjugates e-O1/O139-antibody) kanye nama-conjugate kanogwaja we-IgG-gold, 2) umugqa we-nitrocellulose ulwelwesi oluqukethe amabhendi amabili okuhlola (amabhendi angu-1 nangu-139) kanye nebhendi yokulawula (ibhendi C).Ibhendi engu-1 ifakwe ngaphambili nge-monoclonal anti-V.I-Cholera O1 antibody.Ibhendi ye-139 ifakwe ngaphambili nge-monoclonal anti-V.I-Cholera O139 antibody.Ibhendi C imbozwe ngaphambili nge-anti-mouse IgG antibody.
Uma ivolumu eyanele yesampula yokuhlola isetshenziswa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.V. I-antigen ye-Cholera O1/O139 uma ikhona kusifanekiso izobophezela ku-conjugate yegolide ye-O1/O139-antibody ehambisanayo.Le immunocomplex ibe isithathwa kulwelwesi yi-anti-V eboshwe ngaphambili.I-antibody yekholera i-O1/O139, eyenza ibhendi yokuhlola enombala obomvana, ebonisa umphumela wokuhlolwa oqondile weCholera O1/O139.Ukungabikho kwebhendi yokuhlola kuphakamisa umphumela omubi.
Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-anti-mouse IgG/ mouse IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala ebhendini yokuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.
