Ikhithi Yokuhlola Esheshayo ye-Chlamydia Pneumoniae IgG/IgM (Colloidal Gold)

I-Chlamydia pneumoniae (C. pneumoniae) iwuhlobo olujwayelekile lwebhaktheriya futhi iyimbangela enkulu yenyumoniya emhlabeni jikelele.Cishe u-50% wabantu abadala unobufakazi bokuthi bangenwe yilesi sifo ngesikhathi beneminyaka engama-20, futhi ukuphinda batheleleke kamuva ekuphileni kuvamile.Ucwaningo oluningi luye lwaphakamisa ukuhlobana okuqondile phakathi kokutheleleka kwe-C. pneumoniae nezinye izifo ezivuthayo ezifana ne-atherosclerosis, ukukhushulwa okunamandla kwe-COPD, nesifuba somoya.

Ukuxilonga ukutheleleka kwe-C. pneumoniae kuyinselele ngenxa yemvelo esheshayo ye-pathogen, ukutholakala kwe-seroprevalence enkulu, kanye nethuba lokuhamba kwesikhashana okungabonakali.Izindlela zaselabhorethri zokuxilonga ezimisiwe zihlanganisa ukuhlukaniswa kwento ephilayo kusikompilo yeseli, ukuhlolwa kwe-serological kanye nokuhlolwa kwe-PCR.Microimmunofluorescence (MIF), "izinga legolide" lamanje lokuxilongwa kwe-serological, kodwa ukulinganisa kusantula ukumiswa futhi kuyinselele yobuchwepheshe.Ama-antibody immunoassay ukuhlola okujwayelekile kwe-serology okusetshenziswayo futhi ukutheleleka kwe-chlamydial okuyinhloko kubonakala ngokusabela okuvelele kwe-IgM phakathi kwamaviki ama-2 kuya kwangu-4 kanye nokulibaziseka kwe-IgG ne-IgA phakathi kwamaviki angu-6 kuya kwangu-8.Kodwa-ke, ekuthelelekeni kabusha, amazinga e-IgG ne-IgA akhuphuka ngokushesha, ngokuvamile emasontweni angu-1-2 kuyilapho amazinga e-IgM engase angabonakali.Ngenxa yalesi sizathu, amasosha omzimba e-IgA abonise ukuthi awuphawu oluthembekile lwe-immunological lwezifo eziyinhloko, ezingelapheki neziphindelelayo ikakhulukazi uma kuhlanganiswa nokutholwa kwe-IgM.

Ikhithi yokuhlola esheshayo ye-Chlamydia pneumoniae IgG/IgM isekelwe kumgomo wekhwalithi ye-immunochromatographic assay ukuze kutholwe amasosha omzimba we-Chlamydia pneumoniae IgG/IgM ku-serum yomuntu, i-plasma noma igazi eliphelele. e Antigen conjugates), 2) i-nitrocellulose membrane strip equkethe ibhendi yokuhlola (T band) kanye nebhendi yokulawula (C band).Ibhendi ye-T imbozwe ngaphambili nge-anti-human IgG antibody, futhi ibhande le-C limbozwe ngaphambili nge-anti-mouse IgG antibody.I-strip B iqukethe : 1) i-burgundy colored conjugate pad equkethe i-C. pneumoniae antigen ehlanganiswe negolide eli-colloid (C. pneumoniae Antigen conjugates), 2) a

i-nitrocellulose membrane strip equkethe ibhendi yokuhlola (i-T band) nebhendi yokulawula (i-C band).Ibhendi ye-T imbozwe ngaphambili nge-anti-human IgM antibody, futhi ibhande le-C limbozwe ngaphambili nge-anti-mouse IgG antibody.

Umdweshu A:Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.C.i-pneumoniae IgG antibody uma ikhona kusampula izobophezela ku-C. pneumoniae Antigen conjugates.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgG yegundane ehlanganiswe ngaphambili, yakha ibhendi ye-T enombala o-burgundy,

okubonisa umphumela wokuhlola we-C. pneumoniae IgG.Ukungabikho kwebhendi ye-T kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-anti-mouse IgG/mouse IgGgold conjugate kungakhathaliseki ukuba khona kwebhendi ye-T enombala.Uma kungenjalo, umphumela wokuhlolwa

ayivumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.

Umdweshu B:Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.C.I-antibody ye-pneumoniae IgM uma ikhona kusifanekiso izobophezela ku-C. pneumoniae Antigen conjugates.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgM yegundane ehlanganiswe ngaphambili, yakha ibhendi ye-T enombala onsundu,

okubonisa umphumela wokuhlola we-C. pneumoniae IgM.Ukungabikho kwebhendi ye-T kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-anti-mouse IgG/mouse IgGgold conjugate kungakhathaliseki ukuba khona kwebhendi ye-T enombala.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.