I-Chikungunya IgG/IgM+NS1 Antigen Rapid Test Kit (Colloidal Gold)

I-Chikungunya igciwane legciwane elingavamile elidluliselwa ngokulunywa umiyane osulelekile we-Aedes aegypti.Ibonakala ngokuqubuka, imfiva, nobuhlungu obukhulu bamalunga (arthralgias) obuvamise ukuthatha izinsuku ezintathu kuya kweziyisikhombisa.Leli gama lisuselwa egameni lesiMakonde elisho “lokho okugobayo” kubhekiselwa ekumeni okugobile okuba khona ngenxa yezimpawu zesifo samathambo.Kwenzeka ngesikhathi semvula

ezindaweni ezishisayo zomhlaba, ikakhulukazi e-Afrika, eNingizimu-East Asia, eningizimu yeNdiya nasePakistan.Izimpawu zivame ukubonakala ngendlela engaqondakali ngokomtholampilo lezo ezibonwa kumkhuhlane wodenga.Ngempela, ukutheleleka okukabili kodenga ne-chikungunya kuye kwabikwa eNdiya.Ngokungafani nodenga, ukubonakaliswa ukopha akuvamile futhi ngokuvamile lesi sifo siwumkhuhlane ozikhawulelayo.Ngakho-ke kubaluleke kakhulu

ukuhlukanisa ngokomtholampilo udenga ekuthelelekeni nge-CHIK.I-CHIK ixilongwa ngokusekelwe ekuhlaziyweni kwe-serological kanye nokuhlukaniswa kwegciwane kumagundane noma isiko lezicubu.I-IgM immunoassay iyindlela yokuhlola elebhu esebenza kakhulu.I-Chikungunya IgG/IgM Rapid Test isebenzisa ama-antigen aphindayo atholakala kuphrotheni yesakhiwo sayo, ithola i-IgG/IgM anti-CHIK ku-serum yesiguli noma i-plasma phakathi nemizuzu engu-20.Ukuhlolwa kungenziwa ngabangaqeqeshiwe noma

abasebenzi abanamakhono amancane, ngaphandle kwemishini yaselabhorethri enzima.

I-Chikungunya IgG/IgM+NS1 Antigen Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe i-IgG/IgM Strip kanye ne-NS1 strip.

I-IgG/IgM strip:1) i-burgundy colored conjugate pad equkethe i-Chikungunya recombinant envelope antigens ehlanganiswe negolide le-colloid (dengue conjugates) kanye ne-rabbit IgG-gold conjugates,2) i-nitrocellulose membrane strip equkethe amabhendi amabili okuhlola (amabhendi we-G no-M) kanye ibhendi yokulawula (ibhendi C).Ibhendi ye-G imbozwe ngaphambili nge-anti-Chikungunya ukuze kutholwe igciwane le-IgG anti-Chikungunya, i-M band ifakwe amasosha omzimba ukuze kutholwe igciwane le-IgM elilwa neChikungunya, kanti ibhendi engu-C imbozwe ngaphambili nge-anti-rabbit IgG yembuzi.

NS1 strip:1) i-burgundy colored conjugate pad equkethe i-anti-Chikungunya NS1 antigen yegundane ehlanganiswe negolide eli-colloid (ama-Chikungunya Ab conjugates), 2) umugqa we-nitrocellulose ulwelwesi oluqukethe ibhendi yokuhlola (ibhendi ye-T) kanye nebhendi yokulawula (i-C band).Ibhendi ye-T imbozwe ngaphambili nge-antigen ye-rabbit anti-Chikungunya NS1, futhi ibhande le-C limbozwe ngaphambili nge-anti-mouse IgG antibody.

I-strip ye-IgG/IgM:Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.Igciwane le-IgG anti-Chikungunya uma likhona kusampula lizobophezela kuma-conjugates e-Chikungunya.I-immunocomplex bese ibanjwa yi-reagent ehlanganiswe ku-G band, yakhe ibhande le-G elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-Chikungunya virus we-IgG futhi iphakamisa ukutheleleka kwakamuva noma okuphindaphindiwe.Igciwane le-IgM anti-Chikungunya, uma likhona kusampula, lizobophezela kuma-conjugates e-Chikungunya.I-immunocomplex bese ithathwa yi-reagent embozwe ngaphambili ku-M band, yakhe ibhande le-M elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-Chikungunya virus we-IgM futhi iphakamisa ukutheleleka okusha.Ukungabikho kwanoma yimaphi amabhendi okuhlola (G kanye no-M) kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy le-immunocomplex ye-goat anti rabbit IgG/

i-rabbit IgG-gold conjugate ngaphandle kokuthuthuka kombala kunoma imaphi amabhendi e-T.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.

I-NS1 Strip:Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi lokuhlola.I-Chikungunya NS1 Ag uma ikhona kusifanekiso izobophezela kuma-conjugates e-Chikungunya Ab.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-NS1 antibody kanogwaja eboshwe ngaphambili, yakhe ibhendi ye-T enombala o-burgundy, okubonisa umphumela wokuhlola we-Chikungunya Ag.Ukungabikho kwebhendi ye-T kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-anti-mouse IgG/mouse IgG-gold conjugate kungakhathaliseki ukuba khona kwebhendi ye-T enombala.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.