I-Chikungunya IgG/IgM Rapid Test Kit (Colloidal Gold)

I-Chikungunya igciwane legciwane elingavamile elidluliselwa ngokulunywa umiyane osulelekile we-Aedes aegypti.Ibonakala ngokuqubuka, imfiva, nobuhlungu obukhulu bamalunga (arthralgias) obuvamise ukuthatha izinsuku ezintathu kuya kweziyisikhombisa.Leli gama lisuselwa egameni lesiMakonde elisho “lokho okugobayo” kubhekiselwa ekumeni okugobile okuba khona ngenxa yezimpawu zesifo samathambo.Kwenzeka ngesikhathi sezimvula ezindaweni ezishisayo emhlabeni, ikakhulukazi e-Afrika, eNingizimu-Mpumalanga ye-Asia, eningizimu yeNdiya nasePakistan.Izimpawu zivame ukubonakala ngendlela engaqondakali ngokomtholampilo lezo ezibonwa kumkhuhlane wodenga.Ngempela, ukutheleleka okukabili kodenga ne-chikungunya kuye kwabikwa eNdiya.Ngokungafani nodenga, ukubonakaliswa ukopha akuvamile futhi ngokuvamile lesi sifo siwumkhuhlane ozikhawulelayo.Ngakho-ke kubaluleke kakhulu ukwehlukanisa ngokomtholampilo udenga ekuthelelekeni nge-CHIK.I-CHIK ixilongwa ngokusekelwe ekuhlaziyweni kwe-serological kanye nokuhlukaniswa kwegciwane kumagundane noma isiko lezicubu.I-IgM immunoassay iyindlela yokuhlola elebhu esebenza kakhulu.I-Chikungunya IgG/IgM Rapid Test isebenzisa ama-antigen aphindayo atholakala kuphrotheni yesakhiwo sayo, ithola i-IgG/IgM anti-CHIK ku-serum yesiguli noma ku-plasma phakathi nemizuzu engu-20.Ukuhlolwa kungenziwa ngabangaqeqeshiwe noma

abasebenzi abanamakhono amancane, ngaphandle kwemishini yaselabhorethri enzima.

I-Chikungunya IgG/IgM Rapid Test iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-Chikungunya recombinant envelope antigens ehlanganiswe negolide eli-colloid (ama-dengue conjugates) kanye nama-conjugate kanogwaja we-IgG-gold,2) ulwelwesi lwe-nitrocellulose oluqukethe amabhendi amabili okuhlola (ibhendi ye-G kanye ne-C) nokulawula ibhande).Ibhendi ye-G ifakwe ngaphambili nge-anti-Chikungunya ukuze kutholwe igciwane le-IgG anti-Chikungunya, i-M band ifakwe amasosha omzimba ukuze kutholwe igciwane le-IgM elilwa neChikungunya, kanti ibhendi engu-C imbozwe ngaphambili nge-anti-rabbit IgG yembuzi.

Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.Igciwane le-IgG anti-Chikungunya uma likhona kusampula lizobophezela kuma-conjugates e-Chikungunya.Ama-reagents ezinombolo zamaqoqo ahlukene awakwazi ukusetshenziswa ngokushintshana.I-immunocomplex ibe isithathwa yi-reagent eboshwe ku-G band, yakhe ibhande le-G elinombala o-burgundy, okubonisa umphumela wokuhlola we-Chikungunya virus we-IgG futhi iphakamisa ukutheleleka kwakamuva noma okuphindayo.Igciwane le-IgM anti-Chikungunya, uma likhona kusampula, lizobophezela kuma-conjugates e-Chikungunya.I-immunocomplex bese ithathwa yi-reagent embozwe ngaphambili ku-M band, yakhe ibhande le-M elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-Chikungunya virus we-IgM futhi iphakamisa ukutheleleka okusha.Ukungabi bikho kwanoma yimaphi amabhendi okuhlola (G kanye no-M) kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma imaphi amabhendi e-T.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.